vector pegfp n1 Search Results


pegfp-mmp-9 plasmid  (Genechem)
90
Genechem pegfp-mmp-9 plasmid
Pegfp Mmp 9 Plasmid, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pegfp-n1 plasmid  (BioVector Inc)
90
BioVector Inc pegfp-n1 plasmid
Pegfp N1 Plasmid, supplied by BioVector Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp-n1 plasmid/product/BioVector Inc
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pegfp-n1 plasmid - by Bioz Stars, 2026-03
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pegfp-δcmv-n1 vector  (Promega)
90
Promega pegfp-δcmv-n1 vector
Transcription factor IRF1 Binding Domain (BD) is crucial for ATG10S promotion of IFNL2 transcription. (A) Diagrams of LP1-1-GFP constructs with IRF1-BD deletion (ΔBD) and four pairs of oligonucleotide deletions in the BD. All of the mutants were cloned into <t>pEGFP-ΔCMV-N1</t> (GFP as a reporter gene) and pGL3-basic (luciferase gene as a reporter gene) vectors. (B) GFP expression directed by LP1-1 and LP1-1-ΔBD in HepG2 cells via co-transfected with ATG10 or ATG10S mRNA were analyzed by RT-PCR (upper) and western blotting (bottom), Ctrl, non-transfection control. (C) The luciferase activity driven by LP1-1 and LP1-1-ΔBD under co-transfected with ATG10 or ATG10S mRNA were examined by luciferase assay. (D–F) ATG10/ATG10S-mediated activations of the oligonucleotide-deleted LP1-1 mutants (LP1-1-BDΔ1, LP1-1-BDΔ1-2, LP1-1-BDΔ2-3, and LP1-1-BDΔ3) compared with LP1-1 were detected using RT-PCR (D), western blotting (E), and luciferase assay (F). RLU, relative light unit. * indicates P < 0.05, *** P < 0.001. All of the statistics data are expressed as the mean ± SD (n = 3)
Pegfp δcmv N1 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp-δcmv-n1 vector/product/Promega
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pegfp-δcmv-n1 vector - by Bioz Stars, 2026-03
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pegfp-n1 shrew1 vector  (Amaxa)
90
Amaxa pegfp-n1 shrew1 vector
Transcription factor IRF1 Binding Domain (BD) is crucial for ATG10S promotion of IFNL2 transcription. (A) Diagrams of LP1-1-GFP constructs with IRF1-BD deletion (ΔBD) and four pairs of oligonucleotide deletions in the BD. All of the mutants were cloned into <t>pEGFP-ΔCMV-N1</t> (GFP as a reporter gene) and pGL3-basic (luciferase gene as a reporter gene) vectors. (B) GFP expression directed by LP1-1 and LP1-1-ΔBD in HepG2 cells via co-transfected with ATG10 or ATG10S mRNA were analyzed by RT-PCR (upper) and western blotting (bottom), Ctrl, non-transfection control. (C) The luciferase activity driven by LP1-1 and LP1-1-ΔBD under co-transfected with ATG10 or ATG10S mRNA were examined by luciferase assay. (D–F) ATG10/ATG10S-mediated activations of the oligonucleotide-deleted LP1-1 mutants (LP1-1-BDΔ1, LP1-1-BDΔ1-2, LP1-1-BDΔ2-3, and LP1-1-BDΔ3) compared with LP1-1 were detected using RT-PCR (D), western blotting (E), and luciferase assay (F). RLU, relative light unit. * indicates P < 0.05, *** P < 0.001. All of the statistics data are expressed as the mean ± SD (n = 3)
Pegfp N1 Shrew1 Vector, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp-n1 shrew1 vector/product/Amaxa
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pegfp-n1 shrew1 vector - by Bioz Stars, 2026-03
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cisd1 overexpression vector (pegfp-n1-cisd1  (Promega)
90
Promega cisd1 overexpression vector (pegfp-n1-cisd1
Transcription factor IRF1 Binding Domain (BD) is crucial for ATG10S promotion of IFNL2 transcription. (A) Diagrams of LP1-1-GFP constructs with IRF1-BD deletion (ΔBD) and four pairs of oligonucleotide deletions in the BD. All of the mutants were cloned into <t>pEGFP-ΔCMV-N1</t> (GFP as a reporter gene) and pGL3-basic (luciferase gene as a reporter gene) vectors. (B) GFP expression directed by LP1-1 and LP1-1-ΔBD in HepG2 cells via co-transfected with ATG10 or ATG10S mRNA were analyzed by RT-PCR (upper) and western blotting (bottom), Ctrl, non-transfection control. (C) The luciferase activity driven by LP1-1 and LP1-1-ΔBD under co-transfected with ATG10 or ATG10S mRNA were examined by luciferase assay. (D–F) ATG10/ATG10S-mediated activations of the oligonucleotide-deleted LP1-1 mutants (LP1-1-BDΔ1, LP1-1-BDΔ1-2, LP1-1-BDΔ2-3, and LP1-1-BDΔ3) compared with LP1-1 were detected using RT-PCR (D), western blotting (E), and luciferase assay (F). RLU, relative light unit. * indicates P < 0.05, *** P < 0.001. All of the statistics data are expressed as the mean ± SD (n = 3)
Cisd1 Overexpression Vector (Pegfp N1 Cisd1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cisd1 overexpression vector (pegfp-n1-cisd1/product/Promega
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cisd1 overexpression vector (pegfp-n1-cisd1 - by Bioz Stars, 2026-03
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pegfp-n1 vector  (Kodak)
90
Kodak pegfp-n1 vector
Transcription factor IRF1 Binding Domain (BD) is crucial for ATG10S promotion of IFNL2 transcription. (A) Diagrams of LP1-1-GFP constructs with IRF1-BD deletion (ΔBD) and four pairs of oligonucleotide deletions in the BD. All of the mutants were cloned into <t>pEGFP-ΔCMV-N1</t> (GFP as a reporter gene) and pGL3-basic (luciferase gene as a reporter gene) vectors. (B) GFP expression directed by LP1-1 and LP1-1-ΔBD in HepG2 cells via co-transfected with ATG10 or ATG10S mRNA were analyzed by RT-PCR (upper) and western blotting (bottom), Ctrl, non-transfection control. (C) The luciferase activity driven by LP1-1 and LP1-1-ΔBD under co-transfected with ATG10 or ATG10S mRNA were examined by luciferase assay. (D–F) ATG10/ATG10S-mediated activations of the oligonucleotide-deleted LP1-1 mutants (LP1-1-BDΔ1, LP1-1-BDΔ1-2, LP1-1-BDΔ2-3, and LP1-1-BDΔ3) compared with LP1-1 were detected using RT-PCR (D), western blotting (E), and luciferase assay (F). RLU, relative light unit. * indicates P < 0.05, *** P < 0.001. All of the statistics data are expressed as the mean ± SD (n = 3)
Pegfp N1 Vector, supplied by Kodak, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp-n1 vector/product/Kodak
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pegfp-n1 vector - by Bioz Stars, 2026-03
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living colors vector enhanced green fluorescence protein gene (pegfp)-n1  (Becton Dickinson)
90
Becton Dickinson living colors vector enhanced green fluorescence protein gene (pegfp)-n1
Transcription factor IRF1 Binding Domain (BD) is crucial for ATG10S promotion of IFNL2 transcription. (A) Diagrams of LP1-1-GFP constructs with IRF1-BD deletion (ΔBD) and four pairs of oligonucleotide deletions in the BD. All of the mutants were cloned into <t>pEGFP-ΔCMV-N1</t> (GFP as a reporter gene) and pGL3-basic (luciferase gene as a reporter gene) vectors. (B) GFP expression directed by LP1-1 and LP1-1-ΔBD in HepG2 cells via co-transfected with ATG10 or ATG10S mRNA were analyzed by RT-PCR (upper) and western blotting (bottom), Ctrl, non-transfection control. (C) The luciferase activity driven by LP1-1 and LP1-1-ΔBD under co-transfected with ATG10 or ATG10S mRNA were examined by luciferase assay. (D–F) ATG10/ATG10S-mediated activations of the oligonucleotide-deleted LP1-1 mutants (LP1-1-BDΔ1, LP1-1-BDΔ1-2, LP1-1-BDΔ2-3, and LP1-1-BDΔ3) compared with LP1-1 were detected using RT-PCR (D), western blotting (E), and luciferase assay (F). RLU, relative light unit. * indicates P < 0.05, *** P < 0.001. All of the statistics data are expressed as the mean ± SD (n = 3)
Living Colors Vector Enhanced Green Fluorescence Protein Gene (Pegfp) N1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/living colors vector enhanced green fluorescence protein gene (pegfp)-n1/product/Becton Dickinson
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living colors vector enhanced green fluorescence protein gene (pegfp)-n1 - by Bioz Stars, 2026-03
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pegfp-n1 vector containing halo7-tag sequence  (Kazusa Genome Technologies)
90
Kazusa Genome Technologies pegfp-n1 vector containing halo7-tag sequence
Transcription factor IRF1 Binding Domain (BD) is crucial for ATG10S promotion of IFNL2 transcription. (A) Diagrams of LP1-1-GFP constructs with IRF1-BD deletion (ΔBD) and four pairs of oligonucleotide deletions in the BD. All of the mutants were cloned into <t>pEGFP-ΔCMV-N1</t> (GFP as a reporter gene) and pGL3-basic (luciferase gene as a reporter gene) vectors. (B) GFP expression directed by LP1-1 and LP1-1-ΔBD in HepG2 cells via co-transfected with ATG10 or ATG10S mRNA were analyzed by RT-PCR (upper) and western blotting (bottom), Ctrl, non-transfection control. (C) The luciferase activity driven by LP1-1 and LP1-1-ΔBD under co-transfected with ATG10 or ATG10S mRNA were examined by luciferase assay. (D–F) ATG10/ATG10S-mediated activations of the oligonucleotide-deleted LP1-1 mutants (LP1-1-BDΔ1, LP1-1-BDΔ1-2, LP1-1-BDΔ2-3, and LP1-1-BDΔ3) compared with LP1-1 were detected using RT-PCR (D), western blotting (E), and luciferase assay (F). RLU, relative light unit. * indicates P < 0.05, *** P < 0.001. All of the statistics data are expressed as the mean ± SD (n = 3)
Pegfp N1 Vector Containing Halo7 Tag Sequence, supplied by Kazusa Genome Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp-n1 vector containing halo7-tag sequence/product/Kazusa Genome Technologies
Average 90 stars, based on 1 article reviews
pegfp-n1 vector containing halo7-tag sequence - by Bioz Stars, 2026-03
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pegfp-n1 vector  (BIO-CAT Inc)
90
BIO-CAT Inc pegfp-n1 vector
Transcription factor IRF1 Binding Domain (BD) is crucial for ATG10S promotion of IFNL2 transcription. (A) Diagrams of LP1-1-GFP constructs with IRF1-BD deletion (ΔBD) and four pairs of oligonucleotide deletions in the BD. All of the mutants were cloned into <t>pEGFP-ΔCMV-N1</t> (GFP as a reporter gene) and pGL3-basic (luciferase gene as a reporter gene) vectors. (B) GFP expression directed by LP1-1 and LP1-1-ΔBD in HepG2 cells via co-transfected with ATG10 or ATG10S mRNA were analyzed by RT-PCR (upper) and western blotting (bottom), Ctrl, non-transfection control. (C) The luciferase activity driven by LP1-1 and LP1-1-ΔBD under co-transfected with ATG10 or ATG10S mRNA were examined by luciferase assay. (D–F) ATG10/ATG10S-mediated activations of the oligonucleotide-deleted LP1-1 mutants (LP1-1-BDΔ1, LP1-1-BDΔ1-2, LP1-1-BDΔ2-3, and LP1-1-BDΔ3) compared with LP1-1 were detected using RT-PCR (D), western blotting (E), and luciferase assay (F). RLU, relative light unit. * indicates P < 0.05, *** P < 0.001. All of the statistics data are expressed as the mean ± SD (n = 3)
Pegfp N1 Vector, supplied by BIO-CAT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pegfp-n1-il21 expression vector  (Shanghai Genechem Ltd)
90
Shanghai Genechem Ltd pegfp-n1-il21 expression vector
The expression of FBXL20, PTEN, and <t>IL-21</t> in normal tissues (Con), laryngeal cancer tissues [8] , and adjacent tissues (PT).
Pegfp N1 Il21 Expression Vector, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp-n1-il21 expression vector/product/Shanghai Genechem Ltd
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pegfp-n1-il21 expression vector - by Bioz Stars, 2026-03
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gfpmut1 variant of the gfp-gene30 from the pegfp-n1 vector  (Becton Dickinson)
90
Becton Dickinson gfpmut1 variant of the gfp-gene30 from the pegfp-n1 vector
The expression of FBXL20, PTEN, and <t>IL-21</t> in normal tissues (Con), laryngeal cancer tissues [8] , and adjacent tissues (PT).
Gfpmut1 Variant Of The Gfp Gene30 From The Pegfp N1 Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfpmut1 variant of the gfp-gene30 from the pegfp-n1 vector/product/Becton Dickinson
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gfpmut1 variant of the gfp-gene30 from the pegfp-n1 vector - by Bioz Stars, 2026-03
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pegfp-n1/lsd1 vector, wrl68/ lsd1+/+ cell  (PackGene Biotech lnc)
90
PackGene Biotech lnc pegfp-n1/lsd1 vector, wrl68/ lsd1+/+ cell
The expression of FBXL20, PTEN, and <t>IL-21</t> in normal tissues (Con), laryngeal cancer tissues [8] , and adjacent tissues (PT).
Pegfp N1/Lsd1 Vector, Wrl68/ Lsd1+/+ Cell, supplied by PackGene Biotech lnc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp-n1/lsd1 vector, wrl68/ lsd1+/+ cell/product/PackGene Biotech lnc
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Image Search Results


Transcription factor IRF1 Binding Domain (BD) is crucial for ATG10S promotion of IFNL2 transcription. (A) Diagrams of LP1-1-GFP constructs with IRF1-BD deletion (ΔBD) and four pairs of oligonucleotide deletions in the BD. All of the mutants were cloned into pEGFP-ΔCMV-N1 (GFP as a reporter gene) and pGL3-basic (luciferase gene as a reporter gene) vectors. (B) GFP expression directed by LP1-1 and LP1-1-ΔBD in HepG2 cells via co-transfected with ATG10 or ATG10S mRNA were analyzed by RT-PCR (upper) and western blotting (bottom), Ctrl, non-transfection control. (C) The luciferase activity driven by LP1-1 and LP1-1-ΔBD under co-transfected with ATG10 or ATG10S mRNA were examined by luciferase assay. (D–F) ATG10/ATG10S-mediated activations of the oligonucleotide-deleted LP1-1 mutants (LP1-1-BDΔ1, LP1-1-BDΔ1-2, LP1-1-BDΔ2-3, and LP1-1-BDΔ3) compared with LP1-1 were detected using RT-PCR (D), western blotting (E), and luciferase assay (F). RLU, relative light unit. * indicates P < 0.05, *** P < 0.001. All of the statistics data are expressed as the mean ± SD (n = 3)

Journal: Autophagy

Article Title: A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells

doi: 10.1080/15548627.2020.1719681

Figure Lengend Snippet: Transcription factor IRF1 Binding Domain (BD) is crucial for ATG10S promotion of IFNL2 transcription. (A) Diagrams of LP1-1-GFP constructs with IRF1-BD deletion (ΔBD) and four pairs of oligonucleotide deletions in the BD. All of the mutants were cloned into pEGFP-ΔCMV-N1 (GFP as a reporter gene) and pGL3-basic (luciferase gene as a reporter gene) vectors. (B) GFP expression directed by LP1-1 and LP1-1-ΔBD in HepG2 cells via co-transfected with ATG10 or ATG10S mRNA were analyzed by RT-PCR (upper) and western blotting (bottom), Ctrl, non-transfection control. (C) The luciferase activity driven by LP1-1 and LP1-1-ΔBD under co-transfected with ATG10 or ATG10S mRNA were examined by luciferase assay. (D–F) ATG10/ATG10S-mediated activations of the oligonucleotide-deleted LP1-1 mutants (LP1-1-BDΔ1, LP1-1-BDΔ1-2, LP1-1-BDΔ2-3, and LP1-1-BDΔ3) compared with LP1-1 were detected using RT-PCR (D), western blotting (E), and luciferase assay (F). RLU, relative light unit. * indicates P < 0.05, *** P < 0.001. All of the statistics data are expressed as the mean ± SD (n = 3)

Article Snippet: The 3 units were deleted from the 5ʹ-terminal or 3ʹ-terminal sequentially and inserted into the pEGFP-ΔCMV-N1 vector and pGL3.0-basic vector (Promega, E1751), forming four truncated mutants: BDΔ1, BDΔ1-2, BDΔ2-3 , and BDΔ3 ( ).

Techniques: Binding Assay, Construct, Clone Assay, Luciferase, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Activity Assay

Crucial nucleotides of the IFNL2 LP1-1 segment for ATG10S binding in the core motif (CM) of the IRF1-binding domain. (A) Diagram of CM deletion and six nucleotide-point mutations in the CM. These mutants were cloned into pEGFP-ΔCMV-N1 and pGL3-basic vectors. (B–D) Analysis of transcription activity driven by LP1-1-ΔCM or the point mutation constructs (LP1-1-CM1[C > T], LP1-1-CM2[A > G], LP1-1-CM3[A > C], LP1-1-CM4[G > T], LP1-1-CM5[A > G] or LP1-1-CM6[C > T] mutants) compared with LP1-1 under ATG10 or ATG10S overexpression were detected by RT-PCR (B), western blotting (C), and luciferase assay (D). RLU, relative light unit. *** indicates P < 0.001. All of the data are expressed as the mean ± SD (n = 3). (E) DNA IP analysis of the interactions between ATG10S or ATG10 protein and IFNL2 promoter LP1-1 or LP1-1 DNA mutation fragments (LP1-1-CM1[C > T], LP1-1-CM2[A > G], LP1-1-CM3[A > C], LP1-1-CM4[G > T], LP1-1-CM5[A > G] or LP1-1-CM6[C > T]). These IFNL2 promoter mutants were separately co-transfected with ATG10S or ATG10 into HepG2 cells. The cell lysates were immunoprecipitated with anti-FLAG antibody (FLAG-labeled with ATG10 and ATG10S) and followed PCR determination for these mutated LP1-1 DNAs

Journal: Autophagy

Article Title: A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells

doi: 10.1080/15548627.2020.1719681

Figure Lengend Snippet: Crucial nucleotides of the IFNL2 LP1-1 segment for ATG10S binding in the core motif (CM) of the IRF1-binding domain. (A) Diagram of CM deletion and six nucleotide-point mutations in the CM. These mutants were cloned into pEGFP-ΔCMV-N1 and pGL3-basic vectors. (B–D) Analysis of transcription activity driven by LP1-1-ΔCM or the point mutation constructs (LP1-1-CM1[C > T], LP1-1-CM2[A > G], LP1-1-CM3[A > C], LP1-1-CM4[G > T], LP1-1-CM5[A > G] or LP1-1-CM6[C > T] mutants) compared with LP1-1 under ATG10 or ATG10S overexpression were detected by RT-PCR (B), western blotting (C), and luciferase assay (D). RLU, relative light unit. *** indicates P < 0.001. All of the data are expressed as the mean ± SD (n = 3). (E) DNA IP analysis of the interactions between ATG10S or ATG10 protein and IFNL2 promoter LP1-1 or LP1-1 DNA mutation fragments (LP1-1-CM1[C > T], LP1-1-CM2[A > G], LP1-1-CM3[A > C], LP1-1-CM4[G > T], LP1-1-CM5[A > G] or LP1-1-CM6[C > T]). These IFNL2 promoter mutants were separately co-transfected with ATG10S or ATG10 into HepG2 cells. The cell lysates were immunoprecipitated with anti-FLAG antibody (FLAG-labeled with ATG10 and ATG10S) and followed PCR determination for these mutated LP1-1 DNAs

Article Snippet: The 3 units were deleted from the 5ʹ-terminal or 3ʹ-terminal sequentially and inserted into the pEGFP-ΔCMV-N1 vector and pGL3.0-basic vector (Promega, E1751), forming four truncated mutants: BDΔ1, BDΔ1-2, BDΔ2-3 , and BDΔ3 ( ).

Techniques: Binding Assay, Clone Assay, Activity Assay, Mutagenesis, Construct, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Luciferase, Transfection, Immunoprecipitation, Labeling

The expression of FBXL20, PTEN, and IL-21 in normal tissues (Con), laryngeal cancer tissues [8] , and adjacent tissues (PT).

Journal: Saudi Journal of Biological Sciences

Article Title: Expression and association of IL-21, FBXL20 and tumour suppressor gene PTEN in laryngeal cancer

doi: 10.1016/j.sjbs.2019.08.013

Figure Lengend Snippet: The expression of FBXL20, PTEN, and IL-21 in normal tissues (Con), laryngeal cancer tissues [8] , and adjacent tissues (PT).

Article Snippet: pGPU6/GFP/Neo-FBXL20 siRNA and pEGFP-N1-IL21 expression vectors were constructed by Shanghai GeneChem Co, Ltd, and the oligonucleotides containing the sequence silencing the FBXL20 were inserted into the pGPU6/GFP/Neo-FBXL20 siRNA vector.

Techniques: Expressing

Expression levels of FBXL20, PTEN and IL-21 in HEp-2 cells before and after transfection with pGPU6/GFP/Neo-FBXL20 siRNA plasmids.

Journal: Saudi Journal of Biological Sciences

Article Title: Expression and association of IL-21, FBXL20 and tumour suppressor gene PTEN in laryngeal cancer

doi: 10.1016/j.sjbs.2019.08.013

Figure Lengend Snippet: Expression levels of FBXL20, PTEN and IL-21 in HEp-2 cells before and after transfection with pGPU6/GFP/Neo-FBXL20 siRNA plasmids.

Article Snippet: pGPU6/GFP/Neo-FBXL20 siRNA and pEGFP-N1-IL21 expression vectors were constructed by Shanghai GeneChem Co, Ltd, and the oligonucleotides containing the sequence silencing the FBXL20 were inserted into the pGPU6/GFP/Neo-FBXL20 siRNA vector.

Techniques: Expressing, Transfection

Expression levels of FBXL20, PTEN and IL-21 in HEp-2 cells before and after transfection with pEGFP-N1-IL21 plasmids.

Journal: Saudi Journal of Biological Sciences

Article Title: Expression and association of IL-21, FBXL20 and tumour suppressor gene PTEN in laryngeal cancer

doi: 10.1016/j.sjbs.2019.08.013

Figure Lengend Snippet: Expression levels of FBXL20, PTEN and IL-21 in HEp-2 cells before and after transfection with pEGFP-N1-IL21 plasmids.

Article Snippet: pGPU6/GFP/Neo-FBXL20 siRNA and pEGFP-N1-IL21 expression vectors were constructed by Shanghai GeneChem Co, Ltd, and the oligonucleotides containing the sequence silencing the FBXL20 were inserted into the pGPU6/GFP/Neo-FBXL20 siRNA vector.

Techniques: Expressing, Transfection

pGPU6/GFP/Neo-FBXL20 siRNA and pEGFP-N1-IL21 plasmid transfection suppressed the growth of laryngeal cancer cells.

Journal: Saudi Journal of Biological Sciences

Article Title: Expression and association of IL-21, FBXL20 and tumour suppressor gene PTEN in laryngeal cancer

doi: 10.1016/j.sjbs.2019.08.013

Figure Lengend Snippet: pGPU6/GFP/Neo-FBXL20 siRNA and pEGFP-N1-IL21 plasmid transfection suppressed the growth of laryngeal cancer cells.

Article Snippet: pGPU6/GFP/Neo-FBXL20 siRNA and pEGFP-N1-IL21 expression vectors were constructed by Shanghai GeneChem Co, Ltd, and the oligonucleotides containing the sequence silencing the FBXL20 were inserted into the pGPU6/GFP/Neo-FBXL20 siRNA vector.

Techniques: Plasmid Preparation, Transfection